Course on C. elegans

Kurs/utbildning

To provide participants with fundamental theory regarding C. elegans as a model orgnanism and direct hands-on experience with essential C. elegans methods. The aim is to equip PhD students with the knowledge and skills that they will need to set up a worm activity in their own research group, no matter what that group currently works on.

A One-Week Training on C. elegans

Note: All participants should select a C. elegans paper they would like to present and discuss for the Friday Journal club. Pick a paper that is relevant for yourself.

Monday
10.00: Theory: Worm anatomy, life cycle, cultivation
13.00-17.00 Practice: Make worm plates, make chemotaxis plates.
Worm picking.
Set up a cross of him-5 males with unc-32 hermaphrodites.
Put worms on RNAi plates.

Tuesday
10.00: Theory: Development/Transgenics
13.00-16.00: Practice: Chemotaxis assay.

Wednesday
10.00: Theory: Forward genetics
13.00-15.00: Practice: Mount GFP transgenic worms and observe GFP.

Thursday
10.00: Theory: Reverse genetics and RNAi
13.00-15.00: Practice: Microinjection demonstration.

Friday
10.00: Theory: Journal club
13.00-15.00: Practice: Score RNAi, score cross.

Resources
The following websites are excellent sources of C. elegans information:

www.wormbase.org
www.wormatlas.org
www.wormbook.org

A good chapter regarding worm genetics is found at this specific link: http://www.wormbook.org/toc_wormmethods.html